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- Type of Document: Ph.D. Dissertation
- Language: Farsi
- Document No: 53642 (06)
- University: Sharif University of Technology
- Department: Chemical and Petroleum Engineering
- Advisor(s): Mousavi, Abbas; Abdekhodaie, Mohammad Jafar
- Abstract:
- Stroke is the second most frequent cause of death and the leading cause of disability in the world. Some effective drugs have been developed for ischemic stroke, while the golden time of use is three hours. Therefore, if stroke is diagnosed quickly, its side effects can be greatly avoided. However, it is not possible to use conventional diagnostic methods in many cases at a short time. In this work, S100β, MMP-9 and D-Dimer biomarkers were selected for rapid diagnosis of stroke based on previous studies.For this purpose, electrochemical immunosensors were developed with advantages such as low cost, ease of use, the possibility to automating the device, suitable sensitivity, reproducibility, and fast response. Nanoparticles and nanorods of zinc oxide were used as an intermediate material for the biosensor. Zinc oxide nanorods were prepared utilizing the hydrothermal method. The fabricated electrodes were structurally characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), and infrared spectroscopy (IR).By using zinc oxide nanorods, label-free electrochemical biosensors based on cyclic voltammetry (CV) were developed for S100β, MMP-9 and D-Dimer biomarkers with a sensitivity of 40 µA/(decade.cm2), 32.5 µA/(decade.cm2), and 20 µA/(decade.cm2), respectively. Also, the fabricated biosensors were evaluated for human serum samples by the ELISA method, and no significant difference was observed between the obtained concentration by the presented biosensors in this study with the ELISA method. Ultraviolet light was also used in the electrochemical measurement step, which increased the biosensors' sensitivity by up to 40%. Finally, using the electrodeposition method, silver nanoparticles were deposited on the electrode surface, which could increase the sensitivity of the biosensors by more than 10 times. Furthermore, the limit of detection of biosensors were improved from 0.04 pg/ml, 0.15 ng/ml, and 0.029 ng/ml to 0.002 pg/ml, 0.02 ng/ml, and 0.003 ng/ml, respectively
- Keywords:
- Biosensor ; Biomarker ; Fast Detection ; Stroke ; Immunosensor ; Ischemic Stroke
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