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Degradation of phenanthrene and anthracene by Nocardia otitidiscaviarum strain TSH1, a moderately thermophilic bacterium

Zeinali, M ; Sharif University of Technology | 2008

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  1. Type of Document: Article
  2. DOI: 10.1111/j.1365-2672.2008.03753.x
  3. Publisher: 2008
  4. Abstract:
  5. Aims: The metabolism of phenanthrene and anthracene by a moderate thermophilic Nocardia otitidiscaviarum strain TSH1 was examined. Methods and Results: When strain TSH1 was grown in the presence of anthracene, four metabolites were identified as 1,2-dihydroxy-1,2-dihydroanthracene, 3-(2-carboxyvinyl)naphthalene-2-carboxylic acid, 2,3-dihydroxynaphthalene and benzoic acid using gas chromatography-mass spectrometry (GC-MS), reverse phase-high performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC). Degradation studies with phenanthrene revealed 2,2′-diphenic acid, phthalic acid, 4-hydroxyphenylacetic acid, o-hydroxyphenylacetic acid, benzoic acid, a phenanthrene dihydrodiol, 4-[1-hydroxy(2-naphthyl)]-2-oxobut-3-enoic acid and 1-hydroxy-2-naphthoic acid (1H2NA), as detectable metabolites. Conclusions: Strain TSH1 initiates phenanthrene degradation via dioxygenation at the C-3 and C-4 or at C-9 and C-10 ring positions. Ortho-cleavage of the 9,10-diol leads to formation of 2,2′-diphenic acid. The 3,4-diol ring is cleaved to form 1H2NA which can subsequently be degraded through o-phthalic acid pathway. Benzoate does not fit in the previously published pathways from mesophiles. Anthracene metabolism seems to start with a dioxygenation at the 1 and 2 positions and ortho-cleavage of the resulting diol. The pathway proceeds probably through 2,3-dicarboxynaphthalene and 2,3-dihydroxynaphthalene. Degradation of 2,3-dihydroxynaphthalene to benzoate and transformation of the later to catechol is a possible route for the further degradation of anthracene. Significance and Impact of the Study: For the first time, metabolism of phenanthrene and anthracene in a thermophilic Nocardia strain was investigated. © 2008 The Authors
  6. Keywords:
  7. 1-hydroxy-2-naphthoic acid ; 1,2-dihydroxy-1,2-dihydroanthracene ; 2,2'-diphenic acid ; 2,3-naphthalenediol ; 3-(2-carboxyvinyl)naphthalene-2-carboxylic acid ; 4-[1-hydroxy(2-naphthyl)]-2-oxobut-3-enoic acid ; 4-hydroxyphenylacetic acid ; Anthracene ; Anthracene derivative ; Benzoic acid ; Butyric acid derivative ; Carboxylic acid derivative ; Naphthalene derivative ; Naphthol derivative ; Phenanthrene ; Phenol derivative ; Phthalic acid ; Biodegradation ; Ester ; Gas chromatography ; Mass spectrometry ; Metabolism ; Microbial activity ; Thermophilic bacterium ; Bacterial strain ; Degradation ; Metabolite ; Oxygenation ; Reversed phase high performance liquid chromatography (RP-HPLC) ; Thin layer chromatography (TLC) ; Anthracenes ; Benzoates ; Biodegradation, environmental ; Catechols ; Gas chromatography-mass spectrometry (GC-MS) ; Hot temperature ; Nocardia ; Oxidation-reduction ; Phenanthrenes ; Soil microbiology ; Soil pollutants ; Species specificity ; Bacteria (microorganisms) ; Nocardia otitidiscaviarum
  8. Source: Journal of Applied Microbiology ; Volume 105, Issue 2 , 11 July , 2008 , Pages 398-406 ; 13645072 (ISSN)
  9. URL: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2008.03753.x