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Modified solvent microextraction with back extraction combined with liquid chromatography-fluorescence detection for the determination of citalopram in human plasma

Bagheri, H ; Sharif University of Technology | 2008

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  1. Type of Document: Article
  2. DOI: 10.1016/j.aca.2008.01.047
  3. Publisher: 2008
  4. Abstract:
  5. A modified solvent microextraction with back extraction method (SME/BE) combined with high performance liquid chromatography and fluorescence detection (HPLC-FD) was developed for the determination of citalopram in human plasma. Extraction process was performed in a home-made total glass vial without using a teflon ring, usually employed in SME/BE. Citalopram was first extracted from 0.5 mL of plasma, modified with sodium hydroxide, into hexane. Back extraction step was then performed into 5.2 μL of 45 mM ammonium formate solution (pH 4) using a GC microsyringe. The extract was subsequently transferred into a liner-like vial and then injected into the HPLC system. An enrichment factor of 150 along with a good sample clean-up was obtained. The calibration curve showed linearity in the range of 1.0-130.0 ng mL-1 with regression coefficient corresponding to 0.992. This range covers therapeutic window and even lower amounts which is important in pharmacokinetic studies. Limits of detection and quantification, based on a signal to noise ratio (S/N) of 3 and 10, were 0.3 and 0.8 ng mL-1, respectively. The method was also applied for the determination of citalopram in plasma samples after oral administration of 40 mg single dose of citalopram. © 2008 Elsevier B.V. All rights reserved
  6. Keywords:
  7. Sodium hydroxide ; Solvent microextraction ; Hexane ; pH effects ; Plasma (human) ; Sodium compounds ; Liquid chromatography ; Acetic acid ethyl ester ; Ammonium formate ; Butyl acetate ; Cyclohexane ; Decane ; Glass ; Octane ; Organic solvent ; Toluene ; Unclassified drug ; Xylene ; Calibration ; Controlled study ; Drug determination ; Equipment design ; Gas chromatography microsyringe ; Human ; Human cell ; Human tissue ; pH measurement ; Priority journal ; Process control ; process design ; Reproducibility ; Sensitivity analysis ; Signal noise ratio ; Single drug dose ; Solvent extraction ; Solvent microextraction with back extraction ; Technical aid ; Teflon sleeve ; Chromatography, High Pressure Liquid ; Citalopram ; Humans ; Hydrogen-Ion Concentration ; Serotonin Uptake Inhibitors ; Solvents ; Spectrometry, Fluorescence
  8. Source: Analytica Chimica Acta ; Volume 610, Issue 2 , 2008 , Pages 211-216 ; 00032670 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0003267008001025