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Biodecolourization and Biodetoxification of Dye-containing Wastewaters from Textile Dyeing by the Native Fungal Strain Phanerochaete Chrysosporium and Trametes Species

Alimohammadi, Fatemeh | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54266 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Borghei, Mehdi; Mosavi, Abas; Ghobadi Nehad, Zahra
  7. Abstract:
  8. Treatment of Wastewater of textile industries has been always challenging due to the high organic matter which is a suitable substrate for the growth of microorganisms. Also, discharge of wastewater into the open water causes water adsorbing more sunlight, raising water temperature and therefor decreasing dissolved oxygen. In addition, the acidity of wastewater decreases the quality of agricultural soil. Among wastewater treatment methods, biological methods are cheaper, environment friendly, and produce less by product. Among all the microorganism responsible for biological treatment, fungi and enzymes are more applicable. Especially white-rot fungi are the most effective microorganism to decolorize of textile wastewater.
    In this study, biodecoulorization of four reactive dyes of Red194, Blue19, Orange122, Yellow201 as well as wastewater of textile industry by utilizing fungi of Phanerochate chrysosporium and Trametes species were examined. Different parameters such as pH, initial concentration of dye, carbon and nitrogen concentration were optimized using design expert software and then detoxification, biosorption and enzymatic biodegradation of dye were studied at optimum condition. Results showed that Trametes sp. comparing the Phanerochate chrysosporium could remove more of the colour due to the higher laccase activity, therefore Trametes sp was used to remove the color of Reactive Red 194 in optimiziation experiments. Results also indicated that Trametes sp. Removed 91.29% of the dye when source of the carbon was glucose which is 1.5 times more than when sucrose and molasses were considered as a carbon source. Biosorption and enzymatic biodegradation by dead and alive fungi were studied. It was shown 91.95% of Reactive Red 194 was removed at optimum condition which 68.15% of that happened due to the enzymatic biodegradation and 23.80% due to the biosorption. Hence it can be concluded that activity of ligninolytic enzymes is the main reason behind the decolorization of textile industry wastewater. Biosorption of dye on the surface of fungi was observed by SEM analysis. Toxicity of treated and untreated synthetic wastewater was evaluated on the seed germination of Triticum aestivum, Securigera securidaca, and Cucumis sativus for 7 days. Results showed that 75 ppm of Reactive Red 194 reduced the length of plumule. Also seed germination of Triticum aestivum reduced 40% because of the dye toxicity. 89.39% of dye in textile wastewater was removed at optimum condition. COD and BOD of wastewater reduced to 51.66% and 44.96%, accordingly after decolorization. In general, these promising results showed that Trametes sp. can be used for treatment of wastewater of textile industry
  9. Keywords:
  10. Decolorization ; Dye Wastewater ; Textile Effluent ; Laccas Enzyme ; Bioabsorption ; Chemical Oxygen Demand (COD) ; Total Organic Carbon (TOC) ; Enzymatic Biodegradation ; Phanerochate Chrysosporium Mold ; Trametes Mpecies Mold

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