Loading...

Biohybrid oxidized alginate/myocardial extracellular matrix injectable hydrogels with improved electromechanical properties for cardiac tissue engineering

Mousavi, A ; Sharif University of Technology | 2021

749 Viewed
  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2021.03.097
  3. Publisher: Elsevier B.V , 2021
  4. Abstract:
  5. Injectable hydrogels which mimic the physicochemical and electromechanical properties of cardiac tissue is advantageous for cardiac tissue engineering. Here, a newly-developed in situ forming double-network hydrogel derived from biological macromolecules (oxidized alginate (OA) and myocardial extracellular matrix (ECM)) with improved mechanical properties and electrical conductivity was optimized. 3-(2-aminoethyl amino) propyltrimethoxysilane (APTMS)-functionalized reduced graphene oxide (Amine-rGO) was added to this system with varied concentrations to promote electromechanical properties of the hydrogel. Alginate was partially oxidized with an oxidation degree of 5% and the resulting OA was cross-linked via calcium ions which was reacted with amine groups of ECM and Amine-rGO through Schiff-base reaction. In situ forming hydrogels composed of 4% w/v OA and 0.8% w/v ECM showed appropriate gelation time and tensile Young's modulus. The electroactive hydrogels showed electrical conductivity in the range of semi-conductors and a suitable biodegradation profile for cardiac tissue engineering. Cytocompatibility analysis was performed by MTT assay against human umbilical vein endothelial cells (HUVECs), and the optimal hydrogel with 25 μg/ml concentration of Amine-rGO showed higher cell viability than that for other samples. The results of this study present the potential of OA/myocardial ECM-based hydrogel incorporated with Amine-rGO to provide a desirable platform for cardiac tissue engineering. © 2021 Elsevier B.V
  6. Keywords:
  7. Graphene oxide ; Nanomaterial ; 3-isocyanatopropyl trimethoxysilane ; Alginic acid ; Biomaterial ; Isocyanic acid derivative ; Silane derivative ; Biocompatibility ; Biodegradation ; Cell viability ; Chemical structure ; Controlled study ; Decellularization ; Heart tissue ; Human ; Human cell ; Human tissue ; MTT assay ; Physical chemistry ; Structure analysis ; Synthesis ; Umbilical vein endothelial cell ; Young modulus ; Cardiac muscle ; Cell culture ; Chemistry ; Cytology ; Drug effect ; Electron microscopy ; Endothelium cell ; Hydrogel ; Infrared spectroscopy ; Mechanics ; Oxidation reduction reaction ; Physiology ; Procedures ; Raman spectrometry ; Tissue scaffold ; Alginates ; Biocompatible Materials ; Cell Survival ; Cells, Cultured ; Electric Conductivity ; Endothelial Cells ; Extracellular Matrix ; Graphite ; Heart ; Humans ; Hydrogels ; Isocyanates ; Mechanical Phenomena ; Microscopy, Electron ; Myocardium ; Oxidation-Reduction ; Silanes ; Spectroscopy, Fourier Transform Infrared ; Spectrum Analysis, Raman ; Tissue Engineering ; Tissue Scaffolds
  8. Source: International Journal of Biological Macromolecules ; Volume 180 , 2021 , Pages 692-708 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0141813021006267?via%3Dihub