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Fabrication of a sensitive colorimetric nanosensor for determination of cysteine in human serum and urine samples based on magnetic-sulfur, nitrogen graphene quantum dots as a selective platform and Au nanoparticles

Afsharipour, R ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.1016/j.talanta.2020.122055
  3. Publisher: Elsevier B.V , 2021
  4. Abstract:
  5. A novel colorimetric nanosensor is reported for the selective and sensitive determination of cysteine using magnetic-sulfur, nitrogen graphene quantum dots (Fe3O4/S, N-GQDs), and gold nanoparticles (Au NPs). Thus, S, N-GQDs was firstly immobilized on Fe3O4 nanoparticles through its magnetization in the presence of Fe3+ in the alkali solution. The prepared Fe3O4/S, N-GQDs were dispersed in cysteine solution resulting in its quick adsorption on the surface of the Fe3O4/S, N-GQDs through hydrogen bonding interaction. Then, Au NPs solution was added to this mixture that after a short time, the color of Au NPs changed from red to blue, the intensity of surface plasmon resonance peak of Au NPs at 530 nm decreased, and a new peak at a higher wavelength of 680 nm appeared. The effective parameters on cysteine quantification were optimized via response surface methodology using the central composite design. Under optimum conditions, the absorbance ratio (A680/A530) has a linear proportionality with cysteine concentration in the range of 0.04–1.20 μmol L−1 with a limit of detection of 0.009 μmol L−1. The fabrication of the reported nanosensor is simple, fast, and is capable of efficient quantification of ultra traces of cysteine in human serum and urine real samples. © 2020 Elsevier B.V
  6. Keywords:
  7. Amino acids ; Color ; Fabrication ; Graphene ; Graphene quantum dots ; Hydrogen bonds ; Iron oxides ; Magnetite ; Nanocrystals ; Nanomagnetics ; Nanoparticles ; Nanosensors ; Plasmons ; Semiconductor quantum dots ; Sulfur ; Sulfur determination ; Surface plasmon resonance ; Central composite designs ; Effective parameters ; Fe3O4 nanoparticles ; Human serum and urine samples ; Hydrogen bonding interactions ; Limit of detection ; Optimum conditions ; Response surface methodology ; Gold nanoparticles ; Metal nanoparticle ; Quantum dot ; Human ; Magnetism ; Colorimetry ; Cysteine ; Gold ; Graphite ; Humans ; Magnetic Phenomena ; Metal Nanoparticles ; Nitrogen ; Quantum Dots
  8. Source: Talanta ; Volume 226 , 2021 ; 00399140 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0039914020313461