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Synthesis of Amine-Functionalized BN Quantum Dots for Active Targeting of SKOV3 Cells and Cell Imaging in a Microfluidics System

Bagheri, Omid | 2023

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 56101 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Simchi, Abdolreza; Mohammadi, Mohammad Reza
  7. Abstract:
  8. The biocompatibility and chemical stability of boron nitride nanomaterials surpass those of carbon nanomaterials. In addition, boron nitride nanomaterials share the same structure as graphene materials, making them a suitable replacement for graphene in biological applications. In this study, SKOV3 cancer cells were actively targeted using amine-functionalized boron nitride quantum dots that were covalently modified by CD133 antibody. Under the condition that they are not conjugated with antibody, these nanoparticles were also incorporated into the microfluidic device designed to image MCF7 cancer cells. These nanoparticles were produced via solvothermal synthesis in an organic solvent (NMP). To create the microfluidic chip, the chip mold was first designed in SOLIDWORKS, and then the PMMA mold was fabricated on a CNC machine. Finally, PDMS and cross-linking agent were injected into the mold to create the microfluidic chip. The developed chip contains 26 wells for cell accumulation and the formation of a cell mass. According to the results of the PL test, the synthesized nanoparticles emitted in the green spectrum. The FTIR test revealed that the surface of nanoparticles were functionalized by amine or hydroxyl, and based on the method used to conjugate them with CD133 antibody, it can be inferred that there is an amine functional group on the nanoparticles' surface. The AFM test revealed that the lateral dimension of the synthesized nanoparticles is between 5-40 nm and 1-3 nm in thickness. According to the results of the cytotoxicity test, amine-functionalized boron nitride quantum dots exhibited negligible toxicity. 36 µg/ml was the optimal concentration. The Annexin assay revealed that antibody-conjugated nanoparticles induce 20% necrosis in SKOV3 cells. 85% of SKOV3 cells were successfully targeted by nanoparticles conjugated with the CD133 antibody. Due to their high biocompatibility, these amine-functionalized boron nitride quantum dots can be used in cell imaging, cancer diagnosis, and drug delivery applications, making them a suitable alternative to graphene quantum dots
  9. Keywords:
  10. Quantum Dots ; Microfluidic System ; Cell Imaging ; Cancer Cells ; Boron-Nitride ; Carbon Nanomaterials

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