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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 56207 (06)
- University: Sharif University of Technology
- Department: Chemical and Petroleum Engineering
- Advisor(s): Roosta Azad, Reza; Yeganeh Sarkandi, Shahin
- Abstract:
- Lipases (triacylglycerol acylhydrolases) are industrial enzymes that have wide applications in the detergent, pharmaceutical and food industries. Pseudomonas aeruginosa strain is known to be suitable for lipase production due to its stability in organic solvents, resistance to temperature and pH. Lipases are usually secreted extracellularly, and its isolation from the culture medium is very important. The most cost-effective separation method is filtration which is more economical than centrifuge. Here, the use of filtration method is important from two aspects of creating clearance and maintaining enzyme activity. The equipment in this research is the filter press, whose initial tests are performed in the Buchner funnel. The use of the filtration method faces limitations due to the small particle size of cell masses. To improve the filtration in this research, the common industrial filter aids was used and they were examined in terms of creating clearance and maintaining enzyme activity. Among the filter aids diatomaceous earth, types of clay, bentonite, perlite, charcoal and igneous rock, perlite filter aid had satisfactory results. During the use of this filter aid in equipping the filter press with felt media, the optical density of the solution reached from 7.5 to 0.13, which is a valuable result compared to the optical density of the original centrifuged solution, which is 0.06. The amount of enzyme recovery in this case was 55% and the enzyme activity was estimated to be 3.66 U/ml.
- Keywords:
- Lipase Enzyme ; Pseudomonas Aeruginosa Bacteria ; Filtration ; Filter Aid ; Enzyme Activity ; Heat Resistance ; Fermentation
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