Investigation of the Role of Biological Processes in Bacterial Transport through Porous Media

Biria, Davoud | 2010

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 40574 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Roostaazad, Reza; Sahebghadam Lotfi, Abbas; Amoozegar, Mohammad Ali
  7. Abstract:
  8. To investigate the role of biological processes in bacterial transport through poroud media, six rod shaped and motile bacteria were isolated from a crude oil sample. A multi stage screening procedure was utilized to select the most appropriate isolate in microbial enhanced oil recovery applications (MEOR). It was identified and registered as Bacillus licheniformis MS3 in GenBank database. After that, the physical properties and chemical structure of its biosurfactant were determined and it was proved to be a new lipopeptide biosurfactant which was called licheniformin. Power law logistic model was utilized to investigate the kinetics growth of the MS3 strain on insoluble carbon sources and the effect of mass transfer limitation was studied through experimental analysis. Chemotactic coefficients of bacillus licheniformis MS3 strain to naphthalene as a chemo-attractant were calculated through capillary assay experiments. Then, the contribution of chemotaxis in bacterial fate and transport through porous media in comparison with several other effective parameters such as ionic strength, biosurfactant concentration and the flow rate of the containing fluid was investigated. To achieve this end, Taguchi interactive experimental design was utilized and the L-9 arrays of experiments were conducted in saturated glass bead columns as porous media. The statistical analysis revealed that the contribution of chemotaxis in bacterial filtration through porous media is significant and equal to 20%
  9. Keywords:
  10. Bacteria ; Porous Media ; Biosurfactant ; Chemotaxis ; Transport Processes

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