A Metabonomics Study of Samples of Different Diseases: Investigation of Linear and Non-Linear Model by Nuclear Magnetic Resonance

Fathi, Fariba | 2012

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 43492 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Tafazzoli, Mohsen
  7. Abstract:
  8. Metabonomics is a quantitative measurement of time dependent metabolic interactions for living systems in response to the pathological or genetic variations. NMR spectroscopy has emerged as a key tool for understanding metabolic processes in living systems. In this project, the study of metabolomics was performed as classification and regression on samples of parkinson’s disease, multiple sclerosis disease, celiac disease and crohn’s disease. In classification part, various methods were applied using optimal parameters.classification methods in Parkinson’s disease, multiple sclerosis disease, celiac disease and crohn’s disease were RF, CART, PLS-DA, RF and RF respectively. Based on the previous studies, the importance of the metabolites selected by classification models and their concentration changes in each disease is scrutinized. As an innovative approach, a model was made for correlation between metabolites obtained by NMR and serum levels of selenium and zinc in regression section. Serum and zinc selenium levels were identified by an atomic absorption spectroscopy. To consider the role and effect of metabolites chosen using RF regression model as important features on serum levels of selenium and zinc, the relationship between selenium and these metabolites was inspected using previous researches. Although in this part extensive studies will be needed to verify our present results.

  9. Keywords:
  10. Parkinson Disease ; Nuclear Magnetic Resonance ; Metabonomic ; MS Disease ; Celiac Disease ; Crohn Disease

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