Nanodiamonds for surface engineering of orthopedic implants: Enhanced biocompatibility in human osteosarcoma cell culture

Mansoorianfar, M ; Sharif University of Technology | 2013

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  1. Type of Document: Article
  2. DOI: 10.1016/j.diamond.2013.10.012
  3. Publisher: 2013
  4. Abstract:
  5. Recently, nanodiamonds have attracted interest in biomedical applications such as drug delivery, targeted cancer therapies, fabrication of tissue scaffolds, and biosensors. We incorporated diamond nanoparticles in alginate-bioactive glass films by electrophoretic process to prepare functional coatings for biomedical implants. Turbidity examination by time-resolved laser transmittance measurement revealed that a stable multi-component aqueous suspension of alginate, bioactive glass and diamond particles could be obtained at concentrations of 0.6, 1.3, and 0.65 g/l, respectively. Uniform films with ~ 5 μm thickness were deposited on 316 stainless steel foils by employing constant field strength of 15 V/cm for 45 s. Scanning electron microscopy and simultaneous thermal analysis showed that the composite films were homogeneous and contained ~ 39 wt.% inorganic particles. In vitro bioactivity assessment in simulated body fluid for 4 weeks and 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay using human osteoblast-like cell line (MG-63) and mouse fibroblast cell line (L929) exhibited enhanced biocompatibility and bioactivity of the composite films. Results show advantages of alginate-nanodiamonds in cell culture, tissue engineering, and pharmaceutical applications
  6. Keywords:
  7. Cell viability ; Nanocomposite ; Nanodiamond ; Polysaccharide ; Biomedical applications ; Electrophoretic process ; Human osteoblast-like cells ; Human osteosarcoma cells ; Pharmaceutical applications ; Simultaneous thermal analysis ; Transmittance measurements ; Alginate ; Bioactive glass ; Biocompatibility ; Cell culture ; Composite films ; Drug delivery ; Electrophoresis ; Medical applications ; Nanocomposites ; Nanodiamonds ; Polysaccharides ; Protective coatings ; Scanning electron microscopy ; Thermoanalysis ; Scaffolds (biology)
  8. Source: Diamond and Related Materials ; Volume: 40 , 2013 , Pages: 107-114 ; 09259635 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0925963513002082