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Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model

Yazdani, Y ; Sharif University of Technology | 2013

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  1. Type of Document: Article
  2. DOI: 10.1007/s10529-013-1198-2
  3. Publisher: 2013
  4. Abstract:
  5. Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01)
  6. Keywords:
  7. Baculovirus expression system (BES) ; Baculovirus expression systems ; Cell culture media ; Ferroportin ; Iron concentrations ; Iron metabolism ; Peritoneal macrophage ; Protease inhibitor ; Cardiovascular system ; Mammals ; Cell culture ; Cation transport protein ; Hamp1 protein, mouse ; Hepcidin ; Metal transporting protein 1 ; Recombinant protein ; Animal model ; Biosynthesis ; Blood ; Drug effect ; Genetics ; Macrophage ; Metabolism ; Mouse ; Animals ; Cation Transport Proteins ; Cell Line ; Hepcidins ; Iron ; Macrophages ; Mice ; Models, Animal ; Recombinant Proteins ; Animalia
  8. Source: Biotechnology Letters ; Volume 35, Issue 8 , August , 2013 , Pages 1191-1197 ; 01415492 (ISSN)
  9. URL: http://link.springer.com/article/10.1007%2Fs10529-013-1198-2