Spectrophotometric determination of sulfide based on peroxidase inhibition by detection of purpurogallin formation

Ghadiri, M ; Sharif University of Technology | 2013

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ecoenv.2013.01.015
  3. Publisher: 2013
  4. Abstract:
  5. This paper presents a new method for spectrophotometirc detection of sulfide applying fungal peroxidase immobilized on sodium alginate. The sensing scheme was based on decrease of the absorbance of the orange compound, purpurogallin produced from pyrogallol and H2O2 as substrates, due to the inhibition of peroxidase by sulfide. Absorbance of purpurogallin was detected at 420nm by using a spectrophotometer. The proposed method could successfully detect the sulfide in the concentration range of 0.6-7.0μM with a detection limit of 0.4μM. The kinetic parameters of Michaelis-Menten with and without sulfide were also calculated. Possible inhibition mechanism of peroxidase by sulfide was deduced according to the variation of parameters and uncompetitive mechanism was observed with respect to hydrogen peroxide. The current method provides an easy to use method for sulfide detection in water samples
  6. Keywords:
  7. Immobilization ; Enzyme inhibition ; Sulfide detection ; Alginic acid ; Chemical compound ; Hydrogen peroxide ; Peroxidase ; Purpurogallin ; pyrogallol ; Sulfide ; Unclassified drug ; Enzyme activity ; Lmmobilization ; Lnhibition ; Concentration (parameters) ; Controlled study ; Enzyme activity ; Enzyme immobilization ; Fungal peroxidase ; Enzyme mechanism ; Enzyme substrate ; Inhibition kinetics ; Limit of detection ; Michaelis Menten kinetics ; Optical biosensor ; process optimization ; spectrophotometry ; Benzocycloheptenes ; Biosensing Techniques ; Enzyme Activation ; Enzyme Inhibitors ; Hydrogen Peroxide ; Peroxidases ; Sulfides ; Water ; Water Pollutants, Chemical
  8. Source: Ecotoxicology and Environmental Safety ; Volume 91 , 2013 , Pages 117-121 ; 01476513 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0147651313000316