In situ solid-phase microextraction and post on-fiber derivatization combined with gas chromatography-mass spectrometry for determination of phenol in occupational air

Es haghi, A ; Sharif University of Technology | 2012

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  1. Type of Document: Article
  2. DOI: 10.1016/j.aca.2012.01.002
  3. Publisher: Elsevier , 2012
  4. Abstract:
  5. A method based on solid-phase microextraction (SPME) followed by on-fiber derivatization and gas chromatography-mass spectrometry detection (GC-MS) for determination of phenol in air was developed. Three different types of SPME fibers, polar and non-polar poly(dimethylsiloxane) (PDMS) and polyethylene glycol (PEG) were synthesized using sol-gel technology and their feasibility to the sampling of phenol were investigated. Different derivatization reagents for post on-fiber derivatization of phenol were studied. Important parameters influencing the extraction and derivatization process such as type of fiber coating, type and volume of derivatizing reagent, derivatization time and temperature, extraction time, and desorption conditions were investigated and optimized. The developed method is rapid, simple, easy and inexpensive and offers high sensitivity and reproducibility. Under the optimized conditions, the detection limit of the method was 5ngL-1 using selected ion monitoring (SIM) mode. The inter-day and intra-day precisions of the developed method under optimized conditions were below 10%, and the method shows linearity in the range of 20ngL-1 to 500μgL-1with the correlation coefficient of >0.99. The optimized method was applied to the sampling of phenol from some biologics production areas. The compared results obtained using current and standard methods were shown to be satisfactory
  6. Keywords:
  7. Occupational air ; Sol-gel ; Biologics production ; Derivatization reagent ; Derivatizations ; Derivatizing reagents ; Detection limits ; Extraction time ; Fiber coatings ; High sensitivity ; In-situ ; Non-polar ; On-fiber derivatization ; Optimized conditions ; Polydimethylsiloxane PDMS ; Reproducibilities ; Selected ion monitoring ; Sol-gel technology ; Standard method ; Fibers ; Gas chromatography ; Optimization ; Sol-gel process ; Sol-gels ; Phenols ; Dimeticone ; Macrogol ; Reagent ; Accuracy ; Air analysis ; Air sampling ; Controlled study ; Correlation coefficient ; Derivatization ; Desorption ; Feasibility study ; Fiber ; Intermethod comparison ; Ion monitoring ; Limit of detection ; Linear system ; Mass fragmentography ; Material coating ; Priority journal ; Process development ; Process optimization ; Reproducibility ; Sensitivity analysis ; Sol gel synthesis ; Synthesis ; Temperature sensitivity ; Time ; Air ; Air Pollutants ; Dimethylpolysiloxanes ; Gas Chromatography-Mass Spectrometry ; Hydrogen-Ion Concentration ; Phenol ; Polyethylene Glycols ; Reproducibility of Results ; Solid Phase Microextraction ; Temperature
  8. Source: Analytica Chimica Acta ; Volume 742 , 2012 , Pages 17-21 ; 00032670 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0003267012000165