Cell "vision": Complementary factor of protein corona in nanotoxicology

Mahmoudi, M ; Sharif University of Technology | 2012

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  1. Type of Document: Article
  2. DOI: 10.1039/c2nr31185b
  3. Publisher: RSC , 2012
  4. Abstract:
  5. Engineered nanoparticles are increasingly being considered for use as biosensors, imaging agents and drug delivery vehicles. Their versatility in design and applications make them an attractive proposition for new biological and biomedical approaches. Despite the remarkable speed of development in nanoscience, relatively little is known about the interaction of nanoscale objects with living systems. In a biological fluid, proteins associate with nanoparticles, and the amount and the presentation of the proteins on their surface could lead to a different in vivo response than an uncoated particle. Here, in addition to protein adsorption, we are going to introduce concept of cell "vision", which would be recognized as another crucial factor that should be considered for the safe design of any type of nanoparticles that will be used in specific biomedical applications. The impact of exactly the same nanoparticles on various cells is significantly different and could not be assumed for other cells; the possible mechanisms that justify this cellular response relate to the numerous detoxification strategies that any particular cell can utilize in response to nanoparticles. The uptake and defence mechanism could be considerably different according to the cell type. Thus, what the cell "sees", when it is faced with nanoparticles, is most likely dependent on the cell type
  6. Keywords:
  7. Biosensors ; Cells ; Detoxification ; Drug delivery ; Medical applications ; Biological fluids ; Biomedical applications ; Cell types ; Cellular response ; Defence mechanisms ; Design and application ; Drug delivery vehicles ; Imaging agent ; In-vivo ; Living systems ; Nano-scale objects ; Nanotoxicology ; Protein adsorption ; Safe designs ; Cytology ; Nanoparticle ; Protein ; Reactive oxygen metabolite ; Cell strain HEK293 ; Cell strain HepG2 ; Chemistry ; Drug effect ; Fluorescence microscopy ; Genetic procedures ; HeLa cell ; Human ; leukemia cell line ; lysosome ; Metabolism ; Tumor cell line ; Adsorption ; Biosensing Techniques ; Cell Line, Tumor ; Cell Survival ; HEK293 Cells ; HeLa Cells ; Hep G2 Cells ; Humans ; Jurkat Cells ; Lysosomes ; Microscopy, Fluorescence ; Nanoparticles ; Proteins ; Reactive Oxygen Species
  8. Source: Nanoscale ; Volume 4, Issue 17 , 2012 , Pages 5461-5468 ; 20403364 (ISSN)
  9. URL: http://pubs.rsc.org/en/Content/ArticleLanding/2012/NR/c2nr31185b#!divAbstract