Evaluation of radiogallium-labeled, folate-embedded superparamagnetic nanoparticles in fibrosarcoma-bearing mice

Hosseini Salekdeh, S. L ; Sharif University of Technology | 2012

1520 Viewed
  1. Type of Document: Article
  2. DOI: 10.4103/0973-1482.98971
  3. Publisher: 2012
  4. Abstract:
  5. Context: Elevated expression of the folate receptor (FR) occurs in many human malignancies. Thus, folate targeting is widely utilized in drug delivery purposes specially using nano-radioactive agents. Aims: In this work, we report production and biological evaluation of gallium-67 labeled superparamagnetic iron oxide nanoparticles, embedded by folic acid (67 Ga-SPION-folate) complex especially in tumor-bearing mice for tumor imaging studies. Settings and Design: The structure of SPION-folate was confirmed by X-ray diffraction (XRD), transmission electron microscopy (TEM) and foureir transform infrared spectroscopy (FT-IR) analyses. The radiolabeled SPION-folate formation was confirmed by instant thin layer chromatography (ITLC). Tumor induction was performed by the use of poly-aromatic hydrocarbon injection in rodents as reported previously. Materials and Methods: [67 Ga]-SPION-folate was shown to possess a particle size of ≈5-10 nm using instrumental methods followed by ITLC test. Biocompatibility of the compound was investigated using an 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay followed by stability tests and tumor accumulation studies in fibrosarcoma-bearing mice after subcutaneous (s.c.) application. Statistical Analysis Used: All values were expressed as mean standard deviation (mean ±SD) and the data were compared using Student t-test. Statistical significance was defined as P<0.05. Results: [67 Ga]-SPION-folate was prepared by a modified co-precipitation method possessing a particle size of ≈5-10 nm using instrumental methods (>95% radiochemical purity). Biodistribution studies demonstrated tumor:blood, tumor:bone and tumor:muscle ratios of 4.23, 4.98 and 11.54 respectively after 24 h. Conclusions: Due to the nano-scale size and high-penetrative property of the developed folate-containing nano-complex, this system can be an interesting drug delivery modality with therapeutic applications and folate receptor-targeting behavior, while possessing paramagnetic properties for thermotherapy
  6. Keywords:
  7. Biodistribution ; folate ; Ga-67 ; SPION ; 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide ; gallium 67 ; polycyclic aromatic hydrocarbon derivative ; superparamagnetic iron oxide nanoparticle ; superparamagnetic iron oxide nanoparticle folate gallium ga 67 ; unclassified drug ; animal experiment ; animal model ; animal tissue ; biocompatibility ; chemical carcinogenesis ; drug distribution ; drug synthesis ; Fourier transform infrared photoacoustic spectroscopy ; isotope labeling ; mouse ; nonhuman ; thin layer chromatography ; transmission electron microscopy ; X ray diffraction ; Animals ; Cell Line ; Cell Survival ; Fibrosarcoma ; Folic Acid ; Gallium Radioisotopes ; Magnetite Nanoparticles ; Methylcholanthrene ; Mice ; Nanoparticles ; Particle Size ; Radiopharmaceuticals ; Spectroscopy, Fourier Transform Infrared ; Tissue Distribution ; X-Ray Diffraction
  8. Source: Journal of Cancer Research and Therapeutics ; Volume 8, Issue 2 , 2012 , Pages 204-208 ; 09731482 (ISSN)
  9. URL: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2012;volume=8;issue=2;spage=204;epage=208;aulast=Hosseini-Salekdeh