Production of glucoamylase by aspergillus niger under solid state fermentation

Nahid, P ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.5829/idosi.ije.2012.25.01b.01
  3. Abstract:
  4. In this study, Glucoamylase production by Aspergillus niger was investigated under solid state fermentation using low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Several compositions of corn flours were experimented. The best combination of WB in addition of 10% corn flour resulted maximum enzyme activities. Study of the fermentations in flasks revealed 373.3 IU/gds and 41.4 g/l for the enzyme activity and protein concentration respectively under conditions of 30± 1°C, pH 4.7 and time 96 h. Optimum conditions provided by flask experiments were applied to produce the enzyme in a fermenter. A tray type sterilized fermenter was designed (7-lit volume, 2 trays of 450x230x35 mm, and 2 l/min aeration). The fermentations were performed under aseptic conditions and resulted in enzyme activity and protein concentration of 400 IU/gds, 33.3 mg/gds respectively (under 30± 2°C, pH=4.5 ~ 4.7, aeration 2 l/min, 90% relative humidity and time 72 h). The adequate amounts of enzyme activity, protein concentration and CO2 evolution revealed the efficiency of the bioreactor in comparison with flasks
  5. Keywords:
  6. Agricultural engineering ; Aspergillus ; Atmospheric composition ; Bottles ; Carbon dioxide ; Enzyme activity ; Fermenters ; Proteins ; Agricultural process ; Aspergillus niger ; Corn flour ; Enzyme production ; Glucoamylase ; Glucoamylase production ; Low costs ; Optimum conditions ; Protein concentrations ; Rice brans ; Rice flour ; Solid state fermentation ; Wheat bran ; Fermentation
  7. Source: International Journal of Engineering, Transactions B: Applications ; Volume 25, Issue 1 , 2012 , Pages 1-7 ; 1728-144X (ISSN)
  8. URL: http://www.ije.ir/abstract/%7BVolume:25-Transactions:B-Number:1%7D/=1175