Fabrication of Porous Gelatin-based Scaffold and Evaluation of Effect of Gamma Irradiation

Vahidi, Milad | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 47888 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Forounchi, Massoud; Dadbin, Susan
  7. Abstract:
  8. Biocompatible porous polymeric scaffolds provide a suitable 3-D environment for proliferation of stem cells in human body. For instance, growth and proliferation concurrent with differentiation of stem cells in such scaffolds can regenerate the tissues or organs. In this attitude we fabricated a porous Gelatin-based scaffold using both of freeze-drying and freeze-extraction methods.Also, effect fo gamma irradiation on microstructure of scaffolds was investigated. In addition, poly(ethylene glycol) was employed to make the scaffold softer. Moreover, effects of various parameters including freezing temperature, cross-linking agent concentration, gelatin and PEG concentrations and their blending ratio on physical and mechanical properties, swelling ratio, porosity and pore size and degradation rate of samples are investigated. Also, proliferation of human fibroblast stem cells evaluated by cell culture on desired samples to ensure the potential of produced scaffold in wound healing and skin regeneration. Results showed that adding PEG to scaffold increases the water uptake capacity and also mean pore diameter of them and decreases the proliferation of stem cells in scaffold compared with pure gelatin scaffold. In addition, increasing in dose rate of gamma irradiation decreases the mean pore diameter and also decreases the swelling ratio of them. The results illustrateted that the fabricated 5%-Ge/PEG 8:2 scaffold with chemically cross-linked method is a promising candidate for wound healing application
  9. Keywords:
  10. Gelatin ; Biodegradable Polymer ; Gamma Irradiation ; Poly Ethylene Glycol Fumarate (PEGF) ; Wound Healing ; Cellular Porous Scaffold ; Skin Regeneration

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