A novel photometric glucose biosensor based on decolorizing of silver nanoparticles

Tashkhourian, J ; Sharif University of Technology | 2011

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  1. Type of Document: Article
  2. DOI: 10.1016/j.snb.2011.06.002
  3. Publisher: 2011
  4. Abstract:
  5. A novel glucose biosensor based on chromophore (silver nanoparticles) decolorizing for the photometric determination of glucose was developed. Silver nanoparticles are directly synthesized in the sol-gel matrix by a one-step method based on the reduction of the inorganic precursor AgNO3 and were used for the preparation, characterization and calibration of a highly sensitive and cost-effective localized surface plasmon resonance-based glucose biosensor. In the presence of glucose oxidase (GOx) and due to the enzyme-substrate (glucose) reaction, H2O2 was produced and silver nanoparticles in the sol-gel glass have the ability for the decomposition of hydrogen peroxide. Due to the degradation of silver nanoparticles a remarkable change in the localized surface plasmon resonance absorbance strength could be observed which have been monitored as a suitable signal for determination of substrate concentration. Beer's law is obeyed in the range from 50 to 800 mg/L glucose and the limit of detection is 23 mg/L. The proposed optical biosensor has been successfully applied to the determination of glucose in various real samples
  6. Keywords:
  7. Biosensor ; Localized surface plasmon resonance absorbance ; Absorbances ; Beer's law ; Glucose biosensor ; Highly sensitive ; Inorganic precursor ; Limit of detection ; Localized surface plasmon ; Localized surface plasmon resonance ; One-step methods ; Optical bio-sensors ; Real samples ; Silver nanoparticles ; Sol-gel glass ; Sol-gel matrix ; Substrate concentrations ; Chromophores ; Dyes ; Gels ; Glucose ; Glucose oxidase ; Glucose sensors ; Hydrogen peroxide ; Nanoparticles ; Photometry ; Plasmons ; Sol-gel process ; Sol-gels ; Sols ; Surface plasmon resonance ; Silver
  8. Source: Sensors and Actuators, B: Chemical ; Volume 158, Issue 1 , November , 2011 , Pages 185-189 ; 09254005 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0925400511005107