Fabrication of Artificial skin with Electrospun of Nanofiber made of PCL and PVA

Mohseni, Mina | 2014

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 48073 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Naghdabadi, Reza; Vosooghi, Manoochehr; Firoozbakhsh, Keikhosro
  7. Abstract:
  8. The main goal of this project is to fabricate the scaffolds including Polycaprolactone and Polyvinyle alcohol nanofibers in order to use as artificial skin. For this purpose, electrospinning parameters have been optimized for various injection rates and the scaffolds containing different percent of PCL and PVA have been made. By investigating the cell growth on these scaffolds, the one made up PVA/PCL (30% ) has been introduced as the best scaffold. To improve biological properties of chosen scaffold, a layer of collagen was coated. The angiogenesis capacity has been improved by adding Heparin to PVA nanofibers. Based on assay of heparin release, it was shown that Heparin is released within 2-4 weeks (depending on how the drug has been loaded). Furthermore to improve antibacterial properties of the scaffold, Vancomicine has been added and release profile and antibacterial activity of the scaffold were investigated. It was shown that drug releases totally within 1 week and antibacterial activity is increase during the first week
  9. Keywords:
  10. Tissue Engineering ; Electrospinning ; Scaffold ; Artificial Skin ; Wound Healing

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