Continuous separation processes in microfluidic devices experienced a steep rise in attention during the last two decades. Among the different separation processes, liquid-liquid extraction especially benefits from the short molecular diffusion distance and large specific interfacial area, as these are advantageous for effective mass transport. In the present study, glass-based microfluidic devices have been fabricated utilizing laser ablation and wet chemical etching methods then experiments and numerical simulation were carried out to investigate hydrodynamic behavior of fluid flow in Y-junction microfluidic. In order to achieve phase separation at the end of the microchannel, a phase...