Numerical Simulation of Glucose Metabolism and Hepatocyte Viability within a Microfluidic

Nejadnasrollah, Farzam | 2016

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 48467 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Firoozabadi, Bahar
  7. Abstract:
  8. The advent of microfluidics as suitable environments for culturing cells is associated with some challenges such as shear stresses applied on the cells. In fact, hepatocytes lose their function as exposed to high shear stresses similar to other cell types. Moreover, among all factors needed for cell viability, feeding hepatocytes with adequate oxygen is of great importance due to their high demand for oxygen compared the other cells. In this thesis, different types of cultures including 2D and 3D has been used in order that shear stresses would be in allowed range and provision of hepatocytes with sufficient oxygen concentrations has been ensured as well. In addition to supplying hepatocytes with oxygen, the range of its concentration has been adjusted in physiologic value so that it would be practical for further medical experiments.By doing this range of flow rates by which either sufficient oxygen is supplied or shear stress is limted to its allowable value is obtained. Besides, liver, as one of the most vital organs in human body, has many functions including urea production, detoxification and so on such that its injury causes harmful effects on human living. Among these functions is glucose hemostasis that is one of the main roles of liver in human body and its malfunction leads to many diseases related to glucose regulation. To accomplish this, a model describing glucose metabolism in dynamic condition is used. Obtained results demonstrated the fact that the microfluidic is capable of mimicking glucose hemostasis in addition to glycogen synthesis from glucose similar to in vivo environment
  9. Keywords:
  10. Oxygenation ; Shear Stress ; Microfluidic System ; Hepatocyte ; Glucose Metabolism

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