Comparison of transplantation of bone marrow stromal cells (BMSC) and stem cell mobilization by granulocyte colony stimulating factor after traumatic brain injury in rat

Bakhtiary, M ; Sharif University of Technology | 2010

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  1. Type of Document: Article
  2. Publisher: 2010
  3. Abstract:
  4. Background: Recent clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to compare effect of intravenous injection of bone marrow mesenchymal stem cells (BMSC) and bone marrow hematopoietic stem cell mobilization, induced by granulocyte colony stimulating factor (G-CSF), in rats with a cortical compact device. Methods: Forty adult male Wistar rats were injured with controlled cortical impact device and divided randomly into four groups. The treatment groups were injected with 2 × 106 intravenous bone marrow stromal stem cell (n = 10) and also with subcutaneous G-CSF (n = 10) and sham-operation group (n = 10) received PBS and "bromodeoxyuridine (Brdu)" alone, i.p. All injections were performed 1 day after injury into the tail veins of rats. All cells were labeled with Brdu before injection into the tail veins of rats. Functional neurological evaluation of animals was performed before and after injury using modified neurological severity scores (mNSS). Animals were sacrificed 42 days after TBI and brain sections were stained by Brdu immunohistochemistry. Results: Statistically, significant improvement in functional outcome was observed in treatment groups compared with control group (P<0.01). mNSS showed no significant difference between the BMSC and G-CSF-treated groups during the study period (end of the trial). Histological analyses showed that Brdu-labeled (MSC) were present in the lesion boundary zone at 42nd day in all injected animals. Conclusion: In our study, we found that administration of a bone marrowstimulating factor (G-CSF) and BMSC in a TBI model provides functional benefits
  5. Keywords:
  6. Injection ; Stem cells ; Traumatic brain injury (TBI) ; Animal cell ; Animal experiment ; Animal model ; Animal tissue ; Autologous bone marrow transplantation ; Cell culture ; Cell expansion ; cell function ; Cell isolation ; Controlled study ; Disease severity ; Functional assessment ; Hematopoietic stem cell ; Immunoreactivity ; Motor performance ; Nonhuman ; Stem cell mobilization ; Stroma cell ; Treatment response ; Animals ; Bone Marrow Cells ; Bone Marrow Transplantation ; Brain Injuries ; Bromodeoxyuridine ; Granulocyte Colony-Stimulating Factor ; Hematopoietic Stem Cell Mobilization ; Immunohistochemistry ; Injections, Subcutaneous ; Male ; Mesenchymal Stem Cell Transplantation ; Neurologic Examination ; Nissl Bodies ; Psychomotor Performance ; Rats ; Rats, Wistar ; Staining and Labeling ; Stromal Cells ; Treatment Outcome ; Animalia ; Rattus ; Rattus norvegicus
  7. Source: Iranian Biomedical Journal ; Volume 14, Issue 4 , Oct , 2010 , Pages 142-149 ; 1028852X (ISSN)
  8. URL: http://ibj.pasteur.ac.ir/browse.php?a_id=449&sid=1&slc_lang=en