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Purification and characterization of a novel biosurfactant produced by Bacillus licheniformis MS3

Biria, D ; Sharif University of Technology | 2010

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  1. Type of Document: Article
  2. DOI: 10.1007/s11274-009-0246-5
  3. Publisher: 2010
  4. Abstract:
  5. The physical properties and chemical structure of a new biosurfactant (licheniformin) produced by Bacillus licheniformis MS3 were investigated. The purified biosurfactant was identified as a lipopeptide with amino acid sequence of Gly, Ala, Val, Asp, Ser, Gly, Tyr and a lactone linkage between the carboxyl group of Aspargine and hydroxyl group of Tyrosine residue. The fatty acid moiety was attached to N-terminal amino acid residue through an amide bond. The purified licheniformin could lower the surface tension of water from 72 to 38 mN/m at concentrations higher than 15 μg/mL and its relative emulsion volume (EV%) was equal to 36%. It also showed stable surface activity over a wide range of temperature (45-85°C) and pH (3-11)
  6. Keywords:
  7. Biosurfactant ; Emulsification ; Lipopeptide ; Amide bond ; Amino acid residues ; Amino acid sequence ; Bio surfactant ; Carboxyl groups ; Chemical structure ; Hydroxyl groups ; N-terminals ; Stable surfaces ; Tyrosine residues ; Amides ; Amino acids ; Bacteriology ; Biomolecules ; Fatty acids ; Organic acids ; Phosphatases ; Purification ; Surface active agents ; Surface chemistry ; Surface properties ; Surface tension ; Wetting ; Bacillus licheniformis
  8. Source: World Journal of Microbiology and Biotechnology ; Volume 26, Issue 5 , 2010 , Pages 871-878 ; 09593993 (ISSN)
  9. URL: http://link.springer.com/article/10.1007/s11274-009-0246-5