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Performance characterization of a low-cost dual-channel camera-based microarray scanner

Akhoundi, F ; Sharif University of Technology | 2016

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  1. Type of Document: Article
  2. DOI: 10.1109/IranianCEE.2016.7585765
  3. Publisher: Institute of Electrical and Electronics Engineers Inc , 2016
  4. Abstract:
  5. In this paper, we have proposed, designed, implemented, and characterized a low-cost camera-based microarray scanner which is capable of imaging fluorescently-labeled DNA or Protein microarrays. The proposed system is designed to simultaneously measure two different fluorescent dyes using two parallel channels which increase the overall scan speed. We have shown that the wide dynamic range of system makes it able to detect fluorophore densities from 100-106 molecule/μm2. In each capture, a 5.6 mm × 3.7 mm field is imaged on a 22.3 mm × 14.9 mm (18 megapixels) CMOS sensor. Therefore, the microarray can be scanned with ∼ 1μm2 spatial resolution which is high enough to distinguish borders of spots with less than 50μm diameter. To characterize the scanner performance, we have used molecules labeled with fluorescent cyanine dyes, including Cy3 and Cy5; however, the proposed scanner can be modified to detect any other fluorescent dye with emission spectra within 400-800 nm. We believe it is the most cost-efficient microarray scanner ever achieved. © 2016 IEEE
  6. Keywords:
  7. Camera-based scanner ; CCD and CMOS sensor ; Cy3 and Cy5 dyes ; DNA microarray ; Fluorescence microscopy and imaging ; Biochips ; Cameras ; CMOS integrated circuits ; Costs ; Emission spectroscopy ; Fluorescence ; Fluorescence microscopy ; Microarrays ; Molecules ; Scanning ; Camera-based ; CMOS sensors ; DNA micro-array ; Emission spectrums ; Microarray scanner ; Performance characterization ; Protein microarray ; Wide dynamic range ; Bioassay
  8. Source: 24th Iranian Conference on Electrical Engineering, ICEE 2016, 10 May 2016 through 12 May 2016 ; 2016 , Pages 1534-1538 ; 9781467387897 (ISBN)
  9. URL: http://ieeexplore.ieee.org/document/7585765