Biodegradable polyurethane acrylate/HEMA-grafted nanodiamond composites with bone regenerative potential applications: Structure, mechanical properties and biocompatibility

Alishiri, M ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1039/c5ra19669h
  3. Publisher: Royal Society of Chemistry
  4. Abstract:
  5. The present study demonstrates HEMA-grafted nanodiamond (ND-HEMA)/acrylate-terminated polyurethane-acrylate diluents (APUA) composites as promising materials for bone implant applications. Neat APUA and APUA composites containing ND-HEMA at different loadings up to 2 wt% were prepared by an in situ polymerization method. Morphological analysis demonstrated that ND-HEMAs were actually in the form of tightly bound aggregates which led to formation of big agglomerates at a concentration of 2 wt%. It was also suggested that ND-HEMAs were preferentially localized in the continuous soft domain of APUA; however it interacted by both soft and hard domains. Moreover, ND-HEMAs caused considerable phase separation between soft and hard domains as well as increased crystallinity. Maximum improvement in tensile properties of APUA was observed at 1 wt% loading of ND-HEAMs, namely 175% improvement in modulus and 40% increase in strength. The hydrophilic nature of ND-HEMA enhanced water absorption of composites resulted in higher hydrolysis degradation of APUA. In vitro biocompatibility evaluation via culturing human osteosarcoma cells (MG-63 osteoblast-like cell line), demonstrated no adverse effect on the cell viability of samples. Furthermore, the composites showed favorable cell adhesion and growth, and ND-HEMAs did not cause any negative effect on proliferation, ALP production and osteoblast attachment by MG-63 cells compared with neat APUA
  6. Keywords:
  7. Biocompatibility ; Biomechanics ; Bone ; Cell adhesion ; Cell culture ; Cells ; Cytology ; Osteoblasts ; Phase separation ; Polyurethanes ; Water absorption ; Biodegradable polyurethanes ; Bound aggregates ; Human osteosarcoma cells ; Hydrolysis degradations ; In-situ polymerization ; MG-63 osteoblasts ; Morphological analysis ; Polyurethane acrylates ; Nanodiamonds
  8. Source: RSC Advances ; Volume 6, Issue 11 , 2016 , Pages 8743-8755 ; 20462069 (ISSN)
  9. URL: http://pubs.rsc.org/en/Content/ArticleLanding/RA/2016/C5RA19669H#!divAbstract