Design, Simulation and Fabrication of a Centrifugal Microfluidic Platform for Separation and Studying of Circulating Tumor Cells

Akbari, Ali | 2017

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 49590 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Firoozbakhsh, Keikhosrow
  7. Abstract:
  8. In this project a centrifugal, microfluidic platform to the end of separation and studying circulating tumor cells is designed, modelled and analyzed. Circulating tumor cells (CTCs) are specific types of cancer cells which leave the original tumor and enter the blood stream. For the separation of CTCs, a novel anti-body separation technic has been used which targets the specific MCF7 CTCs using their corresponding Ep-CAM antibody. First the Ep-CAM antibody is coated on magnetic nanoparticles through a chemical bonding process. Afterwards the CTCs are exposed to their antibodies, through which they will bind together and form CTCs with magnetic characteristics. Finally through exerting an external magnetic field, the CTCs are separated from healthy cells. For the purpose of further diagnostic studies on the CTCs, they should initially be lyzed through mixing with a lysis agent. This promotes the need for a mixing unit which has been designed and modelled in this project. A novel Mixing Quality parameter is introduced and effective mixing parameters in the centrifugal microfluidic passive setup are analyzed one by one against this parameter, such that the optimum mixing unit is devised in the end. Together, these two operational units build an integrated microfluidic platform which could be employed in cancer diagnostics
  9. Keywords:
  10. Circulating Tumor Cells (CTC) ; Mixer ; Microfluidic System ; Cell Separation ; Mixing Cells Method ; Mixing Process ; Magnetic Nanodot ; Centrifugal Microfluidics ; Cell Separation Operational Unit ; Mixing Operational Unit

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