Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold

Hosseinzadeh, S ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1016/j.msec.2017.02.101
  3. Abstract:
  4. Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and immunocytochemistry (ICC) assay after co-culturing of MSCs and H-keratino on proposed scaffold. Significant enhancement in cell proliferation was detected after cell culturing on the composite type of electrospun scaffold containing B. vulgaris. Moreover, after 14 days of co-culturing process, gene expression results revealed that both composite and non-composite nylon66 electrospun scaffold promote epithelial differentiation compared to mono-cell culturing of H-keratino in terms of several markers as Cytokeratin 10, Cytokeratin 14 and Involucrin and ICC of some dermal proteins like Cytokeratin 14 and Loricrin. To the best of our knowledge, findings of this study will introduce new way for the generation of novel biomaterials for the development of current skin tissue engineering. © 2017
  5. Keywords:
  6. B. vulgaris ; Co-culture ; Nylon66 ; Skin tissue engineering ; Biocompatibility ; Cell culture ; Cell proliferation ; Cells ; Electrospinning ; Flowcharting ; Gene expression ; Keratin ; Plant extracts ; Plants (botany) ; Polymerase chain reaction ; Proteins ; Stem cells ; Tissue ; Tissue engineering ; Tissue regeneration ; Co-cultures ; Electrospun scaffolds ; Mesenchymal stem cell ; Mesenchyme stem cells ; Nanofibrous scaffolds ; Vulgaris ; Scaffolds (biology) ; Plant extract ; Beet ; Chemistry ; Coculture ; Cytology ; Drug effects ; Epithelium cell ; Human ; Keratinocyte ; Materials testing ; Mesenchymal stroma cell ; Metabolism ; Tissue scaffold ; Beta vulgaris ; Cell differentiation ; Cell line ; Coculture techniques ; Epithelial cells ; Gene expression regulation ; Humans ; Keratinocytes ; Materials testing ; Mesenchymal stromal cells ; Nylons ; Plant extracts ; Tissue scaffolds
  7. Source: Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN)
  8. URL: https://www.sciencedirect.com/science/article/pii/S0928493116320203?via%3Dihub