Simple and rapid detection of L-dopa based on in situ formation of polylevodopa nanoparticles

Hormozi Nezhad, M. R ; Sharif University of Technology | 2017

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  1. Type of Document: Article
  2. DOI: 10.1016/j.snb.2016.12.059
  3. Publisher: Elsevier B.V , 2017
  4. Abstract:
  5. Levodopa [L-3, 4-dihydroxyphenylalanine, or L-DOPA] is an important neurotransmitter used for the treatment of neural disorders such as Parkinson's disease. Abnormal L-Dopa concentrations in biological fluids can be used for the evaluation of such diseases. In this work, a rapid and sensitive method for L-DOPA detection has been reported which is based on in situ formation of polylevodopa nanoparticles. Under alkaline conditions, L-DOPA is spontaneously oxidized to its quinone derivative and shows fluorescence properties. The fluorescence signal of the oxidation product has been monitored and used for the determination of L-DOPA in the presence of dopamine, uric acid, ascorbic acid and other interferents. The developed assay shows excellent selectivity towards L-DOPA. The limit of detection (LOD) of this method is evaluated to be 29.0 nM with a linear range of 0.3–100.0 μM. © 2016 Elsevier B.V
  6. Keywords:
  7. L-DOPA ; L-DOPA oxidation ; Polylevodopa nanoparticles ; Ascorbic acid ; Fluorescence ; Neurophysiology ; Organic acids ; Oxidation ; Alkaline conditions ; Fluorescence properties ; Fluorescence signals ; L-3 ,4-dihydroxyphenylalanine ; Limit of detection ; Oxidation products ; Parkinson's disease ; Nanoparticles
  8. Source: Sensors and Actuators, B: Chemical ; Volume 243 , 2017 , Pages 715-720 ; 09254005 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0925400516320226