On the biological performance of graphene oxide-modified chitosan/polyvinyl pyrrolidone nanocomposite membranes: In vitro and in vivo effects of graphene oxide

Mahmoudi, N ; Sharif University of Technology | 2017

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  1. Type of Document: Article
  2. DOI: 10.1016/j.msec.2016.08.063
  3. Publisher: Elsevier Ltd , 2017
  4. Abstract:
  5. Nanofibrous structures that mimic the native extracellular matrix and promote cell adhesion have attracted considerable interest for biomedical applications. In this study, GO-modified nanofibrous biopolymers (GO) were prepared by electrospinning blended solutions of chitosan (80 vol%), polyvinyl pyrrolidone (15 vol%), polyethylene oxide (5 vol%) containing GO nanosheets (0–2 wt%). It is shown that GO nanosheets significantly change the conductivity and viscosity of highly concentrated chitosan solutions, so that ultrafine and uniform fibers with an average diameter of 60 nm are spinnable. The GO-reinforced nanofibers with controlled pore structure exhibit enhanced elastic modulus and tensile strength (150–300%) with a controllable water permeability to meet the required properties of natural skins. Potential use of the GO-modified biocomposites for tissue engineering is demonstrated in mesenchymal stem cell lines extracted from rat's bone marrow. The biocompatibility assay and SEM imaging reveal that the nanofibrous structure promotes the attachment and maintained characteristic cell morphology and viability up to 72 h. In-vivo evaluations in rats show that a faster and more efficient wound closure rate (about 33%) are attained for the 1.5% GO nanofibrous membrane as compared with control (sterile gauze sponges). © 2016 Elsevier B.V
  6. Keywords:
  7. Animal model ; Biological performance ; Composite membrane ; Graphene oxide ; Biocompatibility ; Biopolymers ; Cell adhesion ; Cell culture ; Cell engineering ; Chitin ; Chitosan ; Composite membranes ; Electrospinning ; Medical applications ; Nanofibers ; Nanosheets ; Polyethylene oxides ; Spinning (fibers) ; Stem cells ; Tensile strength ; Tissue engineering ; Biomedical applications ; Graphene oxides ; Nano-composite membranes ; Nanofibrous membranes ; Native extracellular matrix ; Poly vinyl pyrrolidone ; Graphene
  8. Source: Materials Science and Engineering C ; Volume 70 , 2017 , Pages 121-131 ; 09284931 (ISSN)
  9. URL: https://linkinghub.elsevier.com/retrieve/pii/S0928493116309778