The recent breakthroughs in the lab-on-a-chip technology will not be directly used as clinical devices unless the specific applications are carefully tested and certified. As there is a growing trend towards microfluidic devices for biomedical research, an active microfluidic device was developed. Common microfabrication methods were used to develop a microchip to separate and concentrate live and dead cells based on the dielectrophoresis phenomenon. Unique capabilities of the dielectrophoresis phenomenon make it a powerful tool for micro particle manipulation in micro scale. In this research, geometry for the mentioned purpose is designed and optimized. Three-dimensional microelectrodes are...