Design and Fabrication of Centrifugal Microfluidic System to Measure Blood Factors

Mahmoudi Arjmand, Ehsan | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50691 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Saadatmand, Maryam; Eghbal, Manochehr; Bakhtiari, Mohammad Reza
  7. Abstract:
  8. Diabetes mellitus is a global endemic that is rapidly increasing prevalence in both developing and developed countries. Recently, the American Diabetes Association has recommended the hemoglobin A1c as a possible substitute to fasting blood glucose for diagnosis of diabetes because it is an indicator of long-term glycemic control. Also, centrifugal microfluidics systems have a good potential to be used in the point of care testing systems. In this study, two centrifugal microfluidic discs were designed and manufactured in order to separately measure the hemoglobin and hemoglobin A1c in the whole blood. In the first part of the study, the hemoglobin measurement disc consists of two chambers for entering the blood and the Drabkin's reagent, a two-part mixing chamber, and a chamber to measure the absorbance of the sample according to the cyanmethemoglobin method which is based on the iron oxidation in hemoglobin. In the second part of the study, the hemoglobin A1c disc has three inputs for injection of two reagents and a blood sample, three wall valves, a two-part mixing chamber and a chamber to measure the absorbance of the sample based on the immunoturbidimetry method. In the first and second sections, respectively, 11 and 14 blood samples were tested using the manufactured discs, and the results were very congruous with the clinical data. In this study, the hemoglobin and the hemoglobin A1c in whole blood were measured in 68 seconds and 8 minutes with a standard deviation of ± 0.37 and ± 0.36, respectively
  9. Keywords:
  10. Centrifugal Microfluidics ; Diabetes Mellitus Type 1 ; Hemoglobin ; Hemoglobin A1c ; Point of Care Diagnosis Systems

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