Fabrication of a Multi-Layered Scaffold to Be Used in Dermal Wound Healing

Kamali, Ali | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50763 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Asghari, Mohsen
  7. Abstract:
  8. Wound healing by engineered scaffolds is a new step in bio-technology and medical studies in recent years. The goal of the current study is to propose a novel structure for a tissue-engineered scaffold to be used in wound healing. Influenced from the multi-layered structure of natural human skin, the fabricated scaffold consists of two layers to maximize similarity with natural skin. This product is comprised of an electrospun layer made of polycaprolactone and polyvinyl alcohol and a hydrogel layer made of chitosan and gelatin. In order to form a porous medium in the hydrogel layer, freeze-gelation was used instead of freeze drying. The evaluation of fabricated scaffolds was performed by various tests including FTIR spectroscopy, mechanical tension, degradation, water uptake and swelling, drug release, antibacterial, MTT and in vivo tests. Results indicated that among the various mixing ratios of chitosan and gelatin in the hydrogel layer, the chitosan to gelatin ratio of 75/25 was superior from a number of aspects. The addition of the electrospun sheet to the hydrogel increased the ultimate tensile strength and Young’s modulus of the combination by 110 and 133 %, respectively with respect to the hydrogel state. The optimum fabricated hydrogel and its combination with the electrospun cover showed similar performance to a commercial burn product in the in vivo test with second-degree burns
  9. Keywords:
  10. Electrospinning ; Hydrogel ; Tissue Engineering ; Wound Healing ; Skin Regeneration ; Skin Scaffolds ; Freeze Gelation

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