Characterization of Tissue Structure of Natural Scaffolds Made of Rat Kidney

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Rezaiepour, Yashar | 2017

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 50238 (07)
University: Sharif University of Technology
Department: Materials Science and Engineering
Advisor(s): Asgari, Sirius; Kajbafzadeh, Abdolmohammad
Abstract:
Recently, the deficiency of methods like organ donation and medicine made tissue engineering a prospective method in regeneration of tissues. Despite all of its advantageous like any other method, it has its own weak points. Its major disadvantage is the probability of rejection by the host body. Using proper material and controlling synthesis reactions by researchers decreased the rejection of synthetic scaffolds. But in the case of natural scaffolds due to the range of properties, many tests should be applied on the scaffold prior to implantation in order to evaluate functionality in body. In this research, SDS and TritonX100 has been used to decellulize kidney. Then using uniaxial mechanical compression test, elastic modulus and fracture strength has been calculated. And the results is been interpreted and compared to normal kidney. In the next step using scanning electron microscope figures the results of two methods of acellularization has been compared. Using optical microscope with DAPI and H&E stains cell residual and preservation of structure has been evaluated
Keywords:
Tissue Engineering ; Extracellular Matrix ; Soft Tissues Remodeling ; Regenerative Medicine ; Natural Scaffold

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