Construction of scaffolds composed of acellular cardiac extracellular matrix for myocardial tissue engineering

Esmaeili Pourfarhangi, K ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1016/j.biologicals.2018.03.005
  3. Publisher: Academic Press , 2018
  4. Abstract:
  5. High rates of mortality and morbidity stemming from cardiovascular diseases unveil extreme limitations in current therapies despite enormous advances in medical and pharmaceutical sciences. Following myocardial infarction (MI), parts of myocardium undergo irreversible remodeling and is substituted by a scar tissue which eventually leads to heart failure (HF). To address this issue, cardiac patches have been utilized to initiate myocardial regeneration. In this study, a porous cardiac patch is fabricated using a mixture of decellularized myocardium extracellular matrix (ECM) and chitosan (CS). Results of rheological measurements, SEM, biodegradation test, and MTT assay showed that the scaffold composed of 3.5% (w/w) CS and 0.5% ECM has the best potential in providing cardiac progenitor cells (CPCs) with a suitable microenvironmental condition for both attachment and growth. This study demonstrates that the fabricated scaffold is capable of transmitting both mechanical and chemical cues that is native to myocardial tissue and supports efficient growth of the CPCs. © 2018 International Alliance for Biological Standardization
  6. Keywords:
  7. Cardiac progenitor cells ; Cardiac tissue engineering ; Decellularization ; Porous scaffold ; Animal tissue ; Article ; Biodegradation ; Cardiac stem cell ; Cell adhesion ; Cell growth ; Controlled study ; Flow kinetics ; Heart ; Human ; Human cell ; Nonhuman ; Priority journal ; Animal ; Bovine ; Cardiac muscle ; Cardiac myoblast ; Chemistry ; Cytology ; Metabolism ; Tissue scaffold ; Animals ; Cattle ; Chitosan ; Extracellular Matrix ; Humans ; Myoblasts, Cardiac ; Myocardium ; Tissue Engineering ; Tissue Scaffolds
  8. Source: Biologicals ; Volume 53 , 2018 , Pages 10-18 ; 10451056 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S104510561830071X?via%3Dihub