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Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of salmonella typhimurium
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Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of salmonella typhimurium

Ranjbar, S

Nanoporous gold as a suitable substrate for preparation of a new sensitive electrochemical aptasensor for detection of salmonella typhimurium

Ranjbar, S ; Sharif University of Technology | 2018

599 Viewed
  1. Type of Document: Article
  2. DOI: 10.1016/j.snb.2017.08.160
  3. Publisher: Elsevier B.V , 2018
  4. Abstract:
  5. Nowadays, achievement to easy, fast, reliable and cost-effective techniques and tools is one of the most important challenges in detection of microorganisms. Salmonella typhimurium is one of the most important pathogenic bacteria that affect the human health and environmental infections. In this work we report a new, stable, biocompatible and cost-effective platform for construction of an aptasensor based on nanoporous gold (NPG) for detection of S. typhimurium. Nanoporous gold is electrochemically synthesized using Au-Cu alloy at the surface of Au/GCE. Thiol functionalized aptamer is used for effective linking to the surface of NPG/Au/GCE via self-assemble monolayers (SAMs) formation. Morphological and voltammetric characterizations of the fabricated aptasensor were performed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The purposed aptasensor is capable to detect S. typhimurium in a wide linear dynamic range 6.5 × 102 to 6.5 × 108 CFU mL−1 with a LOQ of 6.5 × 101 CFU mL−1 and LOD of 1 CFU mL−1. The prepared aptasensor showed some significant advantages such as sensitivity, selectivity, repeatability and ability to distinguish between live and dead bacteria cells. The prepared biosensor is successfully applied for salmonella detection in real samples such as egg. © 2017 Elsevier B.V
  6. Keywords:
  7. Bacteria ; Binary alloys ; Biocompatibility ; Chemical detection ; Copper alloys ; Cost effectiveness ; Cyclic voltammetry ; Electrochemical impedance spectroscopy ; Energy dispersive spectroscopy ; Gold ; Salmonella ; Scanning electron microscopy ; Aptasensor ; Au Cu alloy ; ELectrochemical detection ; Food analysis ; Nanoporous gold ; Salmonella typhimurium ; Gold alloys
  8. Source: Sensors and Actuators, B: Chemical ; Volume 255 , 2018 , Pages 1536-1544 ; 09254005 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0925400517315927