This study presents the experimental investigation of the microfluidic microbial fuel cell – microbial electrolysis cell coupled system as a bioenergy generator to produce biohydrogen without any external power supply. The effect of microbial fuel cells number (to provide the required potential for microbial electrolysis cell), the type of substrate and its concentration were assessed. The maximum produced hydrogen of 25, 33 and 50 ppm were obtained in flow rate of 200 μl h-1 and glucose concentration of 200 mg l-1 in 1, 2 and 3 stack MFCs, respectively. For urea solution, by combination of 1, 2 and 3 stack MFCs in the flow rate of 200 μl h-1 and the concentration of 800 mg l-1, the maximum...