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Evaluation and Optimization of Physical Properties of Graphene Oxide Nano Flakes to Confront Antibiotic Resistant Bacteria

Najafi, Fahimeh | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51964 (04)
  4. University: Sharif University of Technology
  5. Department: Physics
  6. Advisor(s): Naseri, Naimeh
  7. Abstract:
  8. Antibacterial resistance towards existing antibiotics has become a major problem in medicine and health in recent years. Therefor it has become a challenge for scientists in different fields to find new antibiotics. Graphene and its derivatives, specially Graphene Oxide has gained attention in this area due to their unique physical properties. Graphene Oxide is considered to be a promising 2D nanomaterial for biomedical applications, due to its ease of production, stability in water, unique chemical and mechanical properties and its biocompatibility compared to other nanomaterials. In this study, graphene Oxide was synthesized using modified Hummers’ method. In order to investigate the effect of GO flake size on its antibacterial properties, different samples of Graphene Oxide were made using a combination of centrifuge for separating different flakes by size and sonication for breaking the flakes. The average flake size for these samples was between 100 µm2 and 0.02 µm2 Antibacterial effect of GO on three important Bacteria, Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii was studied using standard microbiological methods. For Staphylococcus aureus, GO with a concentration as low as 8 µg/mL was shown effective. It was demonstrated that antibacterial effect of Graphene Oxide is inversely related to its flake size. The observed mechanism for the antibacterial effect of Graphene Oxide was wrapping of bacteria with GO flakes which inhibits the growth of bacteria. The flake with a size in the same order of the size of bacteria showed an optimum wrapping effect. For Pseudomonas aeruginosa Go showed no antibacterial properties. The Growth of Acinetobacter baumannii was only inhibited when interacting with GO sample with very low flake size
  9. Keywords:
  10. Graphene Oxide ; Antibacterial Properties ; Antibacterial Resistance ; Nanoflakes ; Hummer's Method ; Graphene Nanosheets

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