Using Surface Properties of Immiscible Fluids in Capillary Tubes for Identification and Separation of Cancerous Blood Cells

Alinejad, Amin | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51944 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Ayatollahi, Shahabodin; Vossoughi, Manochehr
  7. Abstract:
  8. Cancer has been known as one of the main reasons for disease-related deaths in the last decades. Early diagnosis could significantly reduce the level of fatality chances. Among the known cancer types, lung cancer is one of the most malignant ones. The common diagnosticmethods are expensive and using high-technology methods; therefore, the introduction of simple and cheap methods is very urgent to detect it. In this project, surface and interfacial tension measurement of cancerous and normal lung cells have been investigated as an easy detection technique. Among the common measurement methods, Pendant Drop and Capillary height techniques have been utilized in this research work. The obtained results for both methods indicated that the surface and interfacial tension of cancerous cells are approximately 11 and 9 (mN/m) lower than normal ones in the best case, respectively; while the pendant drop method demonstrated better results. It is concluded that the high protein expression of cancerous lung cells than normal cells is the main reason for this observation. Furthermore, the effect of passage number on obtained results has been investigated and observed that by an increase of passage number the amount of surface and interfacial tension increases. In addition of surface and interfacial tension analysis, topographic images and force-distance curves obtained by AFM instrument have been discussed. The results showed that normal cells are 1.5 times stiffer than cancerous ones which are obvious from higher young modulus values of normal ones
  9. Keywords:
  10. Lung Cancer ; Surface Tension ; Pendant-Drop Method ; Capillary Tube ; Atomic Force Microscopy (AFM) ; Force-Distance Curve ; Interfacial Tension ; Youngs Modulus

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