Synthesis, Characterization and Optimization of an In situ Forming Hydrogel Derived from Extracellular Matrix for Cardiac Tissue Engineering Applications

Mousavi, Ali | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52333 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Baheiraei, Nafiseh; Pourjavadi, Ali
  7. Abstract:
  8. Myocardial Infarction occurs due to sudden blockage in a coronary artery and causes necrosis of myocardial tissue. Since myocardium is unable to self-regenerate, cardiac tissue engineering has become a promising therapeutic approach for MI treatment by restoring heart function via combination of cells, biomaterials and signaling factors. For this purpose, myocardial extracellular matrix (ECM) is an attractive biomaterial providing better biomimetic for cultured cells. In this project, we synthesized an in situ forming hydrogel derived from myocardial ECM and for improving mechanical and electrical properties of ECM hydrogel we added oxidized alginate (OA) with 5% oxidation degree and APTMS functionalized reduced graphene oxide (Amine-rGO) to this system. OA can be crosslinked via calcium ions and can crosslink ECM and Amine-rGO via Schiff-base reaction. Several tests including FTIR, XRD, RAMAN, AFM, EDAX, FESEM and zeta potential were performed for characterization of Amine-rGO. The results of gelation time and mechanical properties analysis demonstrated that hydrogels with 4% w/v OA have similar young modulus to native myocardium and form gel in the 90 seconds. Electrical conductivity of hydrogels with Amine-rGO was in the range of cardiac tissue engineering applications and these hydrogels also showed great swelling and biodegradation profiles. To optimize Amine-rGO concentration, cell viability test was performed and 25 ppm nanomaterial concentration showed significant higher HUVEC cell viability than other hydrogel samples
  9. Keywords:
  10. In-Situ Forming Hydrogel ; Extracellular Matrix ; Heart Tissue Engineering ; Oxidized Alginate ; Reduced Graphene Oxide

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