Design and Fabrication of a Spiral Microchannel for High Resolution Blood Cell Separation

Mehran, Amir Hossein | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52593 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saeedi, Mohammad Saeed; Taghipoor, Mojtaba
  7. Abstract:
  8. In medical science, preparing a high purity sample of certain kinds of cells plays an important role in studying the characteristics and functionality of cells, detecting in vitro harmful pathogens and helping to find ways of preventing and treating diseases that cause cellular disorders in the human body. The goal of this research is to improve the spiral microchannel with stair-like cross section in order to separate white blood cells from whole blood. First, the efficiency of this method was evaluated numerically, using a developed finite element model. Then, the desired microchannel mold was made using the micromiling method, and we fabricated the chip using soft lithography and pasting the PDMS layer containing the microchannels on a glass. In the next step, chip performance was evaluated practically with inlet flow rates from 2 up to 8 ml/min using three set of samples containing 10 ml blood with 5%, 2% and 1% hematocrits each in order to analyse the isolation efficiency of white blood cells, red blood cells and platelets from whole blood via particle counting at microchip outlet. The results show that white blood cells in 1% hematocrit blood sample are separated from whole blood with an efficiency of 89% with 5 ml/min flow rate which is the best among the other testing conditions.Also red blood cells and platelets are separated from whole blood with 90.4% and 91.3% efficiency respectively
  9. Keywords:
  10. Isolation ; Passive Methods ; Microfluidic System ; Spiral Microchannel ; Blood Cells Counting

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