Development of a sensitive diagnostic device based on zeolitic imidazolate frameworks-8 using ferrocene-graphene oxide as electroactive indicator for pseudomonas aeruginosa detection

Shahrokhian, S ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1021/acssuschemeng.9b01314
  3. Publisher: American Chemical Society , 2019
  4. Abstract:
  5. Since Gram-negative bacteria have a predominant role in nosocomial infections, there are high demands to develop a fast and sensitive method for diagnosis of bacteria in clinical samples. To address this challenge, we designed a novel electrochemical biosensor based on aptamers immobilized in engineered zeolitic imidazolate Framework-8 (ZIFs-8) via EDC-NHS chemistry. Cyclic voltammetry and electrochemical impedance spectroscopy techniques were conducted to monitor the electrochemical characterization. With respect to unique π-πinteractions between aptamer and graphene oxide (GO), the differential pulse voltammetry technique was applied with ferrocene-graphene oxide (Fc-GO) as an electroactive indicator for the detection of Pseudomonas aeruginosa (P. aeruginosa). In the presence of P. aeruginosa, the configuration of the aptamer showed a change and Fc-GO was released from the electrode surface. On the basis of the signal-off strategy, the proposed biosensor exhibits a wide linear dynamic range (from 1.2 × 101 to 1.2 × 107 CFU mL-1) with a low detection limit. The results reveal that the fabricated aptasensor has high potential applicability in the field of monitoring disease therapy and controlling the safety of the clinical sites. © 2019 American Chemical Society
  6. Keywords:
  7. Differential pulse voltammetry ; Electrochemical biosensor ; Graphene oxide ; Pathogen diagnosis ; Pseudomonas aeruginosa ; Zeolitic imidazolate framework-8 ; Bacteria ; Biosensors ; Cyclic voltammetry ; Diagnosis ; Disease control ; Electrochemical impedance spectroscopy ; Electrodes ; Graphene devices ; Organometallics ; Electrochemical biosensor ; Pathogen diagnosis ; Pseudomonas aeruginosa ; Zeolitic imidazolate framework-8 ; Graphene
  8. Source: ACS Sustainable Chemistry and Engineering ; Volume 7, Issue 15 , 2019 , Pages 12760-12769 ; 21680485 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S138614251930455X