Metabolomics analysis of the saliva in patients with chronic hepatitis b using nuclear magnetic resonance: A pilot study

Gilany, K ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.22038/ijbms.2019.36669.8733
  3. Publisher: Mashhad University of Medical Sciences , 2019
  4. Abstract:
  5. Objective(s): Hepatitis B virus infection causes chronic disease such as cirrhosis and hepatocellular carcinoma. The metabolomics investigations have been demonstrated to be related to pathophysiologic mechanisms in many disorders such as hepatitis B infection. The aim of this study was to investigate the saliva metabolic profile of patients with chronic hepatitis B infection and to identify underlying mechanisms as well as potential biomarkers associated with the disease. Materials and Methods: Saliva from 16 healthy subjects and 20 patients with chronic hepatitis B virus were analyzed by nuclear magnetic resonance (NMR). Then, multivariate statistical analysis was performed to identify discriminative metabolites between two groups. Results: A set of metabolites were detected, including propionic acid, putrescine, acetic acid, succinic acid, tyrosine, lactic acid, butyric acid, pyruvic acid, 4-pyridoxic acid and 4-hydroxybenzoic acid, which in combination with one another could accurately distinguish patients from healthy controls. Our results clearly demonstrated altered metabolites are involved in nine metabolic pathways. Conclusion: Metabolomics has the potential to be considered as a novel clinical tool for hepatitis B diagnosis while contributing to a comprehensive understanding of disease mechanisms. © 2019, Mashhad University of Medical Sciences. All rights reserved
  6. Keywords:
  7. Diagnostic biomarkers ; Hepatitis B virus ; Metabolomics ; NMR Saliva ; Acetic acid ; Alanine aminotransferase ; Biological marker ; Butyric acid ; Hydroxybenzoic acid ; Lactic acid ; Phenylalanine ; Propionic acid ; Putrescine ; Pyridoxic acid ; pyridoxine ; pyruvic acid ; Succinic acid ; Virus DNA ; Amino acid metabolism ; Area under the curve ; Article ; Chronic hepatitis B ; Citric acid cycle ; Clinical article ; Controlled study ; Down regulation ; Gluconeogenesis ; Glycolysis ; Human ; Lipid metabolism ; Male ; Metabolic fingerprinting ; Metabolite ; Metabolome ; Mouth flora ; Pilot study ; Proton nuclear magnetic resonance ; Random forest ; Saliva analysis ; Vitamin metabolism
  8. Source: Iranian Journal of Basic Medical Sciences ; Volume 22, Issue 9 , 2019 , Pages 1044-1049 ; 20083866 (ISSN)
  9. URL: http://ijbms.mums.ac.ir/article_13244.html