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Enhanced electrochemical activity of a hollow carbon sphere/polyaniline-based electrochemical biosensor for HBV DNA marker detection

Salimian, R ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1021/acsbiomaterials.8b01520
  3. Publisher: American Chemical Society , 2019
  4. Abstract:
  5. Herein, we present a novel, simple, and ultrasensitive electrochemical DNA (E-DNA) sensor based on hollow carbon spheres (HCS) decorated with polyaniline (PANI). A thiolated 21-mer oligonucleotide, characteristic of HBV DNA, is immobilized via electrodeposited gold nanoparticles on HCS-PANI. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) are used to characterize the electrochemical properties of the prepared nanocomposite. Scanning electron microscopy is employed to investigate the morphological texture of the fabricated modifier. An enhanced intrinsic signal of PANI is probed to evaluate the biosensing ability of the prepared modifier. The proposed biosensor allows for the detection of the target sequences of HBV DNA at a concentration as low as 10 fM (i.e., 109 DNA copies/mL). In addition, this biosensor demonstrated good capability to differentiate between the perfectly matched target oligonucleotide and three nucleotide-mismatched oligonucleotides. Furthermore, the HCS/PANI-based E-DNA sensor indicates highly sensitive detection of HBV DNA in real samples
  6. Keywords:
  7. Gold nanoparticle ; Hollow carbon sphere ; Polyaniline ; Self-redox signal ; Biosensors ; Carbon ; Cyclic voltammetry ; DNA ; DNA sequences ; Gold nanoparticles ; Metal nanoparticles ; Oligonucleotides ; Polyaniline ; Scanning electron microscopy ; Spheres ; Textures ; Viruses ; Electrochemical biosensor ; Hepatitis B virus ; Hollow carbon spheres ; Label-free detection ; Redox signals ; Electrochemical impedance spectroscopy ; Human placenta lactogen ; Nanocomposite ; Oligonucleotide ; Thiol derivative ; Cyclic potentiometry ; Differential pulse voltammetry ; DNA marker ; DNA sequence ; Electrochemical analysis ; Hybridization ; Impedance spectroscopy ; Infrared spectroscopy ; Morphology ; Nonhuman ; Priority journal ; Structure analysis
  8. Source: ACS Biomaterials Science and Engineering ; Volume 5, Issue 5 , 2019 , Pages 2587-2594 ; 23739878 (ISSN)
  9. URL: https://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.8b01520