Isolation of Circulating Tumor Cells Using an Aptamer-Based Microfluidic Device

Abouali, Hesam | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52822 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vosoughi, Manouchehr; Alemzadeh, Iran
  7. Abstract:
  8. Cancer is a major cause of mortality worldwide, with a disease burden estimated to grow over the coming decades. Circulating tumor cells (CTCs) are rare cancer cells released from the primary or metastatic tumors and transported though the peripheral circulatory system to their specific secondary locations. The presence of CTCs in a cancer patient’s blood has been used as a prognostic biomarker, with lower CTC count correlating with greater overall survival. In spite of its clinical potential, the isolation and detection of CTCs has been a challenging task due to its rare presence amongst other blood cells (as low as 1–10 CTCs per billions of blood cells) and variability in terms of both morphological and biochemical markers.Recent developments of microfluidics technology have paved the way for better isolation and characterization of CTCs due to several advantages such as lower sample volume, higher sensitivity and throughput and lesser production cost. In this thesis, a passive micromixer, which has a staggered herringbone design, is simulated by COMSOL Multiphysics and different design parameters are investigated. Comparing the Mixing Index (M.I.) parameter for various conformations an optimum design is obtained. With help of this simulated unit, isolation of CTCs takes place with help of MUC-1 aptamer. This 5TR1-mediated microchannel enhances the efficiency of the previous immunoaffinity method for isolation of CTCs
  9. Keywords:
  10. Circulating Tumor Cells (CTC) ; Micromixer ; Microfluidic System ; Simulation ; Aptamer ; Metastasis

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