Separation of Plasma from Blood Using a Dielectrophoresis-Assisted Microfluidic Device

Shams, Mehran | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52888 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mohammadi, Ali Asghar; Soltanieh, Mohammad
  7. Abstract:
  8. % of human blood makes up of plasma, that this plasma is a rich source of proteins that can be used as biomarkers for a variety of clinical diseases and laboratory research, therefore, it is necessary that the plasma be separate by a high purity percentage. Using a centrifuge It is a common method used to separate plasma from blood, but because it requires large equipment and a large volume of blood, as well as stress caused by centrifugal force, increases the probability of damage to the biomarkers, so it has been tried today that Use methods that are easy to transport and require a small amount of blood. The idea of using the microfluidic device has been put forward for the purpose of using only hydrodynamic force and geometrical parameters to increase the purity and separation of plasma. In this study, in order to increase plasma purity and separation from blood, a microfluidic device consisting of one main microchannel and five lateral channels using dielectrophoresis force was used to separate red blood cells from the plasma stream. Finally, the results showed that when the inlet flow rate was changed from to ml/min, the plasma separation efficiency decreased and the applied voltage increased to reach the maximum plasma purity efficiency, Therefore, considering the flow rate of ml/min and applying a non-uniform electric field at V and a frequency of MHz it reach to the highest purity and separation
  9. Keywords:
  10. Separation ; Red Blood Cell ; Lab-on-a-Chip ; Dielectrophoresis Technique ; Microfluidic System ; Blood Plasma

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