Construction of Scaffold by Electrospinning Method for Use in Skin Tissue Engineering

Heidari Forushani, Parisa | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52919 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran; Vosoughi, Manouchehr
  7. Abstract:
  8. Making artificial skin and skin alternatives is one of the most important areas in tissue engineering. Although much progress has been made so far, there is still no definitive cure for second- and third-degree burns. To create new tissue in the body, a suitable substrate for cells is needed, which is called scaffolding, and an ideal scaffold in tissue engineering should mimic the dimensions of extracellular matrix, and nanofibers seem to be the best option for this purpose. Among the methods of manufacturing of nanofibers, electrospun is very easy and accurate method. In previous studies, many natural and synthetic polymers such as chitosan, alginate, collagen, polyathylene oxide, etc. have been used. In this project, the aim is to make an electrospinning scaffold withnatural polymers like gelatin and silk , which in addition to having mechanical properties and suitable for human skin, also has antibacterial properties and controls and treats wound infections. Natural antibiotics are used for scaffolding in scaffolding. Green method is used to make silver nanoparticles and no chemical compounds are used. In this method, there is no need to separate the nanoparticles and the silver is regenerated by the polymers used and electrified at the same time. After preparing , distributing of the nanoparticles were tested for greater effectiveness, and after electrospinning and scaffolding, to prevent water dissolution, a crosslinking was made in the scaffold by temperature and ethanol, followed by the antibacterial, mechanical, degradability, water absorption and effect of these particles on the release of curcumin were then investigated . The results of this study are as follows: Considering that silk nanofibers have a diameter of about 700 nanometers and gelatin nanofibers have a diameter of about 200 nanometers with simultaneous electrification of these two polymers by two , nozzles with a ratio of 2 to 1 silk to gelatin. Improves the properties of scaffolding compared to pure state, including proper porosity and fiber preparation with an average diameter of about 400 nm, improves the mechanical properties of scaffold ,faster degrade and more suitable for 20 days of skin treatment and proper water absorption and better biocompatibility than using pure mode. Nanoparticles were prepared by two methods of silk reduction and gelatin reduction, the diameter of nanoparticles that was reduced by silk was 100 nanometers, and showed better antibacterial properties. With the addition of nanoparticles in scaffolding, the release of curcumin became slower and more controlled.About 70% of the drug is released on the first 20 days . Adding nanoparticles increases the Yang module of gelatin scaffolding. Due to the fact that no harmful chemicals were used in the preparation of scaffolding and nanoparticles, the results of the cell biocompatibility test were more appropriate than other studies and because the concentration of silver used was in the appropriate range for cells no toxicity was created for the cells. The adhesion of the cells to the scaffolding was very good, and the adhesion of the gelatinscaffold was greater than silk due to the smaller size of the gelatin fibers, and the increase in silver reduced the adhesion
  9. Keywords:
  10. Electrospinning ; Gelatin ; Silver Nanoparticles ; Skin Tissue Engineering ; Wound Healing ; Silk

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