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3D Bioprinting of oxygenated cell-laden gelatin methacryloyl constructs

Erdem, A ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1002/adhm.201901794
  3. Publisher: Wiley-VCH Verlag , 2020
  4. Abstract:
  5. Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of 3D bioprinted tissues. Supplementing oxygen (O2) to transplanted cells via an O2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide that reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant importance. In this study, a gelatin methacryloyl (GelMA) bioink that is optimized in terms of pH and viscosity is developed. The improved rheological properties lead to the production of a robust bioink suitable for 3D bioprinting and controlled O2 release. In addition, O2 release, bioprinting conditions, and mechanical performance of hydrogels having different CPO concentrations are characterized. As a proof of concept study, fibroblasts and cardiomyocytes are bioprinted using CPO containing GelMA bioink. Viability and metabolic activity of printed cells are checked after 7 days of culture under hypoxic condition. The results show that the addition of CPO improves the metabolic activity and viability of cells in bioprinted constructs under hypoxic condition. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
  6. Keywords:
  7. 3D bioprinting ; Bioinks ; Calcium peroxide ; Cardiomyocytes ; Hypoxia ; Oxygen ; 3D printers ; Blood vessels ; Cell culture ; Hydrated lime ; Lime ; Metabolism ; Oxidation ; Peroxides ; Viscosity ; Calcium peroxides ; Cardiomyocytes ; Hypoxic condition ; Mechanical performance ; Metabolic activity ; Proof of concept ; Rheological property ; Transplanted cells ; Cells ; Gelatin ; Gelatin methacryloyl ; Hydrogel ; Peroxide ; Unclassified drug ; Article ; Cardiac muscle cell ; Cell hypoxia ; Cell viability ; Compression stress ; Concentration (parameter) ; Controlled study ; Elasticity ; Fibroblast ; Flow kinetics ; Human ; Human cell ; Mechanical stress ; Metabolic regulation ; PH ; Pore size ; Porosity ; Priority journal ; Scanning electron microscopy ; Three dimensional bioprinting ; Young modulus
  8. Source: Advanced Healthcare Materials ; Volume 9, Issue 15 , 2020
  9. URL: https://onlinelibrary.wiley.com/doi/full/10.1002/adhm.201901794