Graphene oxide negatively regulates cell cycle in embryonic fibroblast cells

Hashemi, E ; Sharif University of Technology | 2020

673 Viewed
  1. Type of Document: Article
  2. DOI: 10.2147/IJN.S260228
  3. Publisher: Dove Medical Press Ltd , 2020
  4. Abstract:
  5. Background: Unique properties of graphene and its derivatives make them attractive in the field of nanomedicine. However, the mass application of graphene might lead to side effects, which has not been properly addressed in previous studies, especially with regard to its effect on the cell cycle. Methods: The effect of two concentrations (100 and 200 μg/mL) of nano-and microsized graphene oxide (nGO and mGO) on apoptosis, cell cycle, and ROS generation was studied. The effect of both sizes on viability and genotoxicity of the embryonic fibroblast cell cycle was evaluated. MTT and flow cytometry were applied to evaluate the effects of graphene oxide (GO) nanosheets on viability of cells. Apoptosis and cell cycle were analyzed by flow cytometry. Results: The results of this study showed that GO disturbed the cell cycle and nGO impaired cell viability by inducing cell apoptosis. Interestingly, both nGO and mGO blocked the cell cycle in the S phase, which is a critical phase of the cell cycle. Upregulation of TP53-gene transcripts was also detected in both nGO-and mGO-treated cells compared to the control, especially at 200 μg/mL. DNA content of the treated cells increased; however, because of DNA degradation, its quality was decreased. Conclusion: In conclusion, graphene oxide at both nano-and micro-scale damages cell physiology and increases cell population in the S phase of the cell cycle. © 2020 Hashemi et al
  6. Keywords:
  7. Apoptosis ; Cell cycle ; Fibroblast cell ; Graphene ; TP53 gene ; Cell DNA ; Graphene oxide ; Nanoparticle ; Protein p53 ; Reactive oxygen metabolite ; Nanomaterial ; Trp53 protein, mouse ; Animal cell ; Article ; Cell cycle regulation ; Cell viability ; Concentration (parameter) ; Controlled study ; DNA content ; DNA degradation ; Embryo cell ; Female ; Fibroblast ; Flow cytometry ; G1 phase cell cycle checkpoint ; Genotoxicity ; MEF cell line ; Mouse ; MTT assay ; Nonhuman ; Particle size ; Animal ; Cell culture ; Cytology ; Drug effect ; Genetics ; Mammalian embryo ; Metabolism ; Mutagen testing ; Physiology ; Animals ; Cell Survival ; Cells, Cultured ; Embryo, Mammalian ; Fibroblasts ; Gene Expression Regulation ; Graphite ; Mice ; Mutagenicity Tests ; Nanostructures ; Reactive Oxygen Species ; Tumor Suppressor Protein p53
  8. Source: International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 6201-6209
  9. URL: https://www.dovepress.com/graphene-oxide-negatively-regulates-cell-cycle-in-embryonic-fibroblast-peer-reviewed-fulltext-article-IJN