A novel pathway to produce biodegradable and bioactive PLGA/TiO2 nanocomposite scaffolds for tissue engineering: Air–liquid foaming

Pelaseyed, S. S ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1002/jbm.a.36910
  3. Publisher: John Wiley and Sons Inc , 2020
  4. Abstract:
  5. Poly (lactate-co-glycolate) (PLGA) is a typical biocompatible and biodegradable synthetic polymer. The addition of TiO2 nanoparticles has shown to improve compressive modulus of PLGA scaffolds and reduced fast degradation. A novel method has been applied to fabricate PLGA/TiO2 scaffolds without using any inorganic solvent, with aim of improving the biocompatibility, macroscale morphology, and well inter-connected pores efficacy: Air–Liquid Foaming. Field Emission Scanning Electron Microscopy (FESEM) revealed an increase in interconnected porosity of up to 98%. As well the compressive testing showed enhancement in modulus. Bioactivity and in vitro degradation were studied with immersion of scaffolds in Simulated Body Fluid (SBF) and incubation in Phosphate Buffered Saline (PBS), respectively. Formation of apatite layer corroborated the bioactivity after soaking in SBF. Degradation rate of scaffolds was increased with excessive addition of TiO2 contents withal. The in vitro cultured human-like MG63 ostoblast cells showed attachment, proliferation, and nontoxcitiy in contact, using MTT assay [3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide]. According to the results, the novel method utilized in this study generated porous viable tissue without using any inorganic solvent or porogen can be a promising candidate in further treatment of orthopedic patients effectively. © 2020 Wiley Periodicals, Inc
  6. Keywords:
  7. Air–liquid foaming ; Bioactivity ; Biocompatibility ; Bioactivity ; Biocompatibility ; Biodegradable polymers ; Biodegradation ; Body fluids ; Degradation ; Field emission microscopes ; Liquids ; Nanocomposites ; Patient treatment ; Phosphate minerals ; Scanning electron microscopy ; TiO2 nanoparticles ; Tissue ; Titanium dioxide ; Well testing ; Compressive testing ; Field emission scanning electron microscopy ; Further treatments ; Interconnected porosity ; Nanocomposite scaffolds ; Phosphate-buffered salines ; PLGA ; Simulated body fluids ; Scaffolds (biology) ; Apatite ; Phosphate buffered saline ; polyglactin ; polymer ; Titanium dioxide nanoparticle ; Biocompatibility ; Biodegradability ; Cell adhesion ; Cell proliferation ; Compression ; Field emission scanning electron microscopy ; Foaming ; Human cell ; In vitro study ; Morphology ; MTT assay ; Porosity ; Tissue engineering ; Nanocomposite
  8. Source: Journal of Biomedical Materials Research - Part A ; Volume 108, Issue 6 , 2020 , Pages 1390-1407
  9. URL: https://pubmed.ncbi.nlm.nih.gov/32108983